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Degenerated Primers and their problems

Degenerated primers pcr problems

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#1 Sampathkumar



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Posted 24 July 2014 - 08:56 AM

Dear Scientists,


         I have designed, degenerated primers but in PCR There is no product. 

can anybody suggest me. how to carryout my PCR. Thanks in advance.


#2 hobglobin


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Posted 24 July 2014 - 10:56 AM

we need a lot of more information (concentrations, pcr program, etc etc) to help you. Degenerated primers are not necessarily a problem, but usually helpful for a pcr. But any badly designed primer can ruin a pcr. Therefore if you think the primer are the problem, you also should give the primer sequences and perhaps the target gene.

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

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