I'm currently growing FRTL-5 cells (rat thyroid cell line). We have recently encountered a problem with these cells after having transfected them. Whereas before they used to be growing in a nice monolayer (prior to transfection), they are now in the forms of clustered spheres.
Would it still be possible for us to detach these cells and re-plate them again. If so, are there any necessary precautions that we should take into account before doing so? Thanks in advance for the help!