Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Reviving SF268 cells

  • Please log in to reply
No replies to this topic

#1 wooz2002



  • Members
  • Pip
  • 3 posts

Posted 23 July 2014 - 12:52 AM

Hi everyone,


I'm trying to revive a vial of SF268 cells (purchased from NCI through a local supplier shipped halfway around the globe) using RPMI with 10% FBS and 2mM L-glutamine in a T25 cell culture flask. I spin-down the original vial content, discarded the supernatant, resuspended the cells with fresh media and added to the flask.


However, after 24 hours all the cells are still floating/in suspension. Are the cells still viable? Should I wait longer to see if they adhere? Any suggestions?


Thank you for the help.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.