hi colleagues
suggest me how to do sscp in pcr fragment size of 655 bp and how much concentration of acrylamide & bisacrylamide can be used in page electrophoresis
Posted 23 July 2014 - 12:00 AM
hi colleagues
suggest me how to do sscp in pcr fragment size of 655 bp and how much concentration of acrylamide & bisacrylamide can be used in page electrophoresis
Posted 30 July 2014 - 07:29 AM
thank u mdm, i need suggestions wrt to single strand confirmation polymorphism in which how much concentration of acrylamide: bis can i use as my pcr product length is 655bp
Posted 01 August 2014 - 07:50 AM
i've never performed sscp but for sequencing and other molecular biology page techniques 19:1 acrylamide/bisacrylamide is generally used.
you may want to try a 5% gel (no more than 6%).
talent does what it can
genius does what it must
i used to do what i got paid to do
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