hi colleagues
suggest me how to do sscp in pcr fragment size of 655 bp and how much concentration of acrylamide & bisacrylamide can be used in page electrophoresis
Submit your paper to J Biol Methods today!
2 replies to this topic
#2
vageesh pandith
-
- Members
-
- 2 posts
member
0
Neutral
Neutral
Posted 30 July 2014 - 07:29 AM
thank u mdm, i need suggestions wrt to single strand confirmation polymorphism in which how much concentration of acrylamide: bis can i use as my pcr product length is 655bp
#3
mdfenko
-
- Active Members
-
- 3,369 posts
an elder emeritus
217
Excellent
Excellent
Posted 01 August 2014 - 07:50 AM
i've never performed sscp but for sequencing and other molecular biology page techniques 19:1 acrylamide/bisacrylamide is generally used.
you may want to try a 5% gel (no more than 6%).
- vageesh pandith likes this
talent does what it can
genius does what it must
i used to do what i got paid to do
