hi colleagues
suggest me how to do sscp in pcr fragment size of 655 bp and how much concentration of acrylamide & bisacrylamide can be used in page electrophoresis
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#2
vageesh pandith
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Posted 30 July 2014 - 07:29 AM
thank u mdm, i need suggestions wrt to single strand confirmation polymorphism in which how much concentration of acrylamide: bis can i use as my pcr product length is 655bp
#3
mdfenko
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Posted 01 August 2014 - 07:50 AM
i've never performed sscp but for sequencing and other molecular biology page techniques 19:1 acrylamide/bisacrylamide is generally used.
you may want to try a 5% gel (no more than 6%).
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