Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

Sonication of small PCR amplicon

Started by ForEpi, Jul 22 2014 11:16 PM

PCR sonication

Please log in to reply

1 reply to this topic

#1 ForEpi

Enthusiast

Active Members
33 posts

0
Neutral

Posted 22 July 2014 - 11:16 PM

Hi colleagues,

what happens to an amplicon of, say, 500bp after 1 round or 2 rounds of shearing? Will I get a majority of intact amplicons and an equal distribution of all other fragment sizes or will there be a tailing off with decreasing length? Anyone have any experience with this?

Best,

Bram

I believe in the power of Selenium!!

#2 Trof

Brain on a stick

Global Moderators
1,349 posts

134
Excellent

Posted 24 July 2014 - 07:23 AM

That is very easy to test.
Make your amplicon, split, shear one part 1 round and the other 2 rounds and put on gel. You will see.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon

Back to General Lab Techniques · Next Unread Topic →

Also tagged with one or more of these keywords: PCR, sonication

Protocols and Techniques Forums → Molecular Biology → Odd issue converting mutiplex PCR to single reactions Started by MealyWorm, 18 Aug 2017 PCR, multiplex PCR, Multiplexing and 2 more...	3 replies 390 views	mdfenko 21 Aug 2017
Protocols and Techniques Forums → PCR, RT-PCR and Real-Time PCR → problem with PCR amplification of cDNA Started by roshni mohan, 05 Aug 2017 PCR, Cloning, cDNA	4 replies 555 views	roshni mohan 17 Aug 2017
Protocols and Techniques Forums → PCR, RT-PCR and Real-Time PCR → Problematic DNA Extraction Started by hmwmx, 14 Apr 2017 extraction, pcr, dna	1 reply 723 views	merlav 26 Apr 2017
Protocols and Techniques Forums → PCR, RT-PCR and Real-Time PCR → Recombinant DNA vaccine production Started by Mashudu, 02 Feb 2017 Primer design, Molecular biology and 3 more...	9 replies 739 views	bob1 14 Feb 2017
Protocols and Techniques Forums → siRNA, microRNA and RNAi → Where can I find 100-200 unique primer sequences for preparation of array Started by Bio21, 16 Jan 2017 shRNA, array, PCR, primer	0 replies 1,024 views	Bio21 16 Jan 2017