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Sonication of small PCR amplicon

PCR sonication

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#1 ForEpi

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Posted 22 July 2014 - 11:16 PM

Hi colleagues,

 

what happens to an amplicon of, say, 500bp after 1 round or 2 rounds of shearing? Will I get a majority of intact amplicons and an equal distribution of all other fragment sizes or will there be a tailing off with decreasing length? Anyone have any experience with this?

 

Best,

 

Bram


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#2 Trof

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Posted 24 July 2014 - 07:23 AM

That is very easy to test.
Make your amplicon, split, shear one part 1 round and the other 2 rounds and put on gel. You will see.


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