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SDS PAGE sample preparation question, and discussion?

electrophoresis SDS PAGE sample preparation

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#1 kyri_osity

kyri_osity

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Posted Yesterday, 02:36 PM

If it is necessary to dilute out an SDS-PAGE sample(s) after adding the protein (to a specific final concentration), the SDS Sample Loading Buffer (final = 1x), why use anything other than pure water?  Each sample, depending on the initial concentration of the protein, could contain different volumes of the dilutent. 

notebook excerpt - SDS PAGE sample prep final concentrations.jpg  

 

If using a buffer, then each sample could have slightly different concentrations from one another.  Why introduce more than one variable in ]between samples?

notebook excerpt - SDS PAGE sample prep.jpg







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