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Viability dye 7-AAD


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#1 Shoooda

Shoooda

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Posted 21 July 2014 - 10:04 AM

Hi,

Does anyone have experience with this dye 7-AAD? I am wondering when to add it to my cells ? i.e. prior to doing my cytofix/cytoperm or at the end of staining with everything. 

I am doing a 10-color panel, which includes three intracellular cytokines (TNF, IFNg, IL-2: obviously needing cytofix/cytoperm) 

Thanks,

Shoooda






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