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Viability dye 7-AAD


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#1 Shoooda

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Posted 21 July 2014 - 10:04 AM

Hi,

Does anyone have experience with this dye 7-AAD? I am wondering when to add it to my cells ? i.e. prior to doing my cytofix/cytoperm or at the end of staining with everything. 

I am doing a 10-color panel, which includes three intracellular cytokines (TNF, IFNg, IL-2: obviously needing cytofix/cytoperm) 

Thanks,

Shoooda



#2 JGBarin

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Posted 11 August 2014 - 09:38 AM

7AAD works on primary live cells, and is not fixable.

 

You add it right before acquiring live cells on the cytometer. It can be used to stain already fixed, permabilized cells for DNA quantitation.

 

For fixable viability dyes, look at Invitrogen/Molecular Probes LIVE/DEAD series - available in a broad spectrum.

 

Similar fixable dyes are available from BioLegend and eBiosciences.



#3 Angeline

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Posted 14 September 2014 - 04:17 PM

Hi. I have the same confusion as well. I wanted to use 7AAD in conjunction with intracellular staining
If stain the cells with 7AAD, then proceed to fixation and permeabilization for intracellular staining? Would that work?

#4 Tabaluga

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Posted 14 September 2014 - 04:40 PM

http://www.ebioscien...bility-dyes.htm


Edited by Tabaluga, 14 September 2014 - 04:43 PM.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 





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