this is a standard 12% SDS PAGE (4% stack) gel run under standard reducing conditions (boiled 95C for 10 minutes in sample reducing buffer)
The samples are blood and plasma.
As you can see the separation is fine until about 80kDa and there are nice defined bands.
After this molecular weight however there are no nice defined bands, just smearing and then those big bands at the bottom.
The molecular weight markers are running fine and I don't think it is a gel issue as I have done this we a pre-made bought gel and with those I have made myself and I am getting the same results.
I'm not really sure what could be happening here
Any suggestions would be great!
Edited by biochem06, 21 July 2014 - 03:47 AM.