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Will S. griseus grow on Columbia blood agar?


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#1 tretol

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Posted 20 July 2014 - 06:19 AM

Hello,

 

I got S. griseus from DSMZ. I dissolved the pellet in LB medium containing 0,3% Tween-80 and stored at 4°C for 2 days. I put onto Columbia sheep blood agar two days ago and on another agar medium (yeast extract, meat extract, glucose, NaCl, Peptone, Tryptone) and incubated at 30°C.

 

Will the bacteria grow under these conditions? Till now there is no visible growth. I am asking because on the DSMZ home page they recommend GYM medium (glucose, yeast extract, malt extract, CaCO3). Two of these ingredients I do not have inside my agars.

I think it should not make a difference and I have incubate for at least 10 days until I can see growth, but I am not sure since I have not used this bacterium berfore.

 

 

What do you think?



#2 pito

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Posted 30 July 2014 - 02:55 AM

it can take a few days, up to 7 if I am not mistaken, but it should grow yes.

But I can be wrong, its been a long time for me.


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#3 El Crazy Xabi

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Posted 30 July 2014 - 05:40 PM

Check the documentation provided by the DSMZ. If they don't state the incubation time, better ask them. They should be able to give you the expected incubation time in a given medium for your specific strain.



#4 Adriana Reis

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Posted 27 August 2014 - 05:33 AM

the documentation provided by DSMZ is almost always non existent. And when we contact them they not always helpful.

give it a go wait 7 days and contact them






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