I got S. griseus from DSMZ. I dissolved the pellet in LB medium containing 0,3% Tween-80 and stored at 4°C for 2 days. I put onto Columbia sheep blood agar two days ago and on another agar medium (yeast extract, meat extract, glucose, NaCl, Peptone, Tryptone) and incubated at 30°C.
Will the bacteria grow under these conditions? Till now there is no visible growth. I am asking because on the DSMZ home page they recommend GYM medium (glucose, yeast extract, malt extract, CaCO3). Two of these ingredients I do not have inside my agars.
I think it should not make a difference and I have incubate for at least 10 days until I can see growth, but I am not sure since I have not used this bacterium berfore.
What do you think?