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Dark LB media


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4 replies to this topic

#1 bradeupland

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Posted 01 July 2004 - 07:35 AM

Hi,
I am new to cloning. My overnight culture looks very dark...almost a hint of blue. Is this from contamination or overgrowth. Thank you for educating the clueless.

#2 rassen

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Posted 01 July 2004 - 09:46 AM

I have never experienced this. When the overnight culture reaches saturation, the color of the media should not be so dark. It would be thicker and cloudy.

Are you sure your LB is freshly prepared and antibiotics is still working?

#3 leahf

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Posted 03 July 2004 - 10:49 AM

Try streaking your overnight culture onto 2 LB plates, with and without antibiotics - contanmination (by anything different from coli) is likely to show up as morphologically different colonies. if you get colonies only in the minus antibiotic plates, then thats the problem with your medium.
Luck
Leah

#4 kawaka

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Posted 03 July 2004 - 06:12 PM

Maybe this is the same problem somebody has mentioned before

http://www.protocol-...p?showtopic=376

#5 Vincent Delespaux

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Posted 04 August 2004 - 05:45 AM

Very dark and blue? Sure there is no IPTG and X gal in your medium?? :(




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