I am doing a small summer project in C. elegans lab and in the first step, I am supposed to find out upstream sequence of a few genes and design primers to amplify that region. Once the primers are designed, I am asked to check if those primers bind to any other region of the genome.
Can anybody direct me on how to find the upstream region of the gene on Wormbase or on any other database. From what I noticed, Wormbase only gives the sequence of the gene and not the upstream region. Please correct me if I am wrong.
And how do I find out that my primer pair is not going to produce any other product BEFORE running a PCR?
Thank you very much.