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Increase in enzyme activity

enzyme activity

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#1 carollee

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Posted 16 July 2014 - 08:52 PM

It is usually a trend to see a decline in enzyme activity as time passes but  I am seeing an increase in enzyme activity after a period of incubation/storage (after the partial purification). Are there any comments about this scenario?



#2 mdfenko

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Posted 17 July 2014 - 04:06 AM

since it's only partially purified there are a few possible reasons why enzyme activity may increase. here are a few which immediately come to mind:

 

partial hydrolysis of the enzyme (may deactivate regulatory subunit or portion of the enzyme or free the active site)

 

conformational changes to the enzyme

 

decomposition of endogenous inhibitor(s)

 

partial aggregation sometimes increases activity (before more complete aggregation reduces activity)


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