Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Increase in enzyme activity

enzyme activity

  • Please log in to reply
1 reply to this topic

#1 carollee



  • Active Members
  • Pip
  • 11 posts

Posted 16 July 2014 - 08:52 PM

It is usually a trend to see a decline in enzyme activity as time passes but  I am seeing an increase in enzyme activity after a period of incubation/storage (after the partial purification). Are there any comments about this scenario?

#2 mdfenko


    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,076 posts

Posted 17 July 2014 - 04:06 AM

since it's only partially purified there are a few possible reasons why enzyme activity may increase. here are a few which immediately come to mind:


partial hydrolysis of the enzyme (may deactivate regulatory subunit or portion of the enzyme or free the active site)


conformational changes to the enzyme


decomposition of endogenous inhibitor(s)


partial aggregation sometimes increases activity (before more complete aggregation reduces activity)

talent does what it can
genius does what it must
i do what i get paid to do

Also tagged with one or more of these keywords: enzyme activity

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.