2 replies to this topic

[leahf]

Hi All
when doing isolation of genomic bacterial DNA  using the phenol extraction method, is it possible to replace the phenol extractions by two phenol:chloroform (1:1) extractions, followed by 1 choloroform extraction? (when i tried this i got unpure dna, but do not know if this is the reason for the problem...)
thanks
leah

[Bioteck]

Hi:
I use fenol:Clor.  For genomic extraction in yeast, but the protocol is similar to bacteria.

[leahf]

Right, thanks.  encouraging to know it does work for someone else!