Hi, guys. I need your help.
I’m culturing H9c2 myoblast, for about month now, which should be passaged at 80% confluency. Till now I’ve been using LUNA cell counting device. But I’m suspicious about received results. According to LUNA, the number of cells harvested from bottles are usually a few thousand (5-8). Funny thing is that results do not change that much when I use T-75 instead of T-25.
So lately I've started to count my cells with use of Thoma chamber. And now I’m getting results around 20x10^6 from T-25 flask. Is that even possible? I’ve got Thoma chamber with 0.2mm x 0.2mm dimension so I multiply the average number of cell from medium squares by dilution factor and 250 000.
Could you tell me how many cells are on average on T-25 flask? Where do I make mistake?