I have tried to express my protein - sucrose synthase from rice, in [/I]P.pastoris. Althuogh I tried both electroporation and " EasyComp" from Invitrogen, I could not get overexpression of my protein.
Here are my inducing conditions:
30 C
150 rpm
medium BMGY FOR 16~18hr
change to BMMY OD600=1
used 0.5% methanol for induction
I am curious that should I try higher methanol for induction or try to incube in different temperature?
Or how can I get an overexpression of my protein?
thanks
