Hi all, I'm new to suspension cell culture, and so is my lab. Labmates are reluctant to buy a new type of untreated culture vessels for my culture, so I wonder if it's OK to use typical surface treated TC flasks/plates for suspension cell culture. Thank you very much.
Do I need to use untreated TC flask for suspension cell culturesuspension cell culture culture vessel
Posted 15 July 2014 - 05:33 PM
Yes, it works OK, but depends a on the cells as to whether they will attach or not, and how this will affect your results.
Posted 16 July 2014 - 05:01 AM
My lab occasionally grows suspension-type cells in tissue culture flasks designed for attached cells. One trick we use is to tip the flask to the vertical (cap up in the air) after seeding to minimize the amount of contact between the suspended cells and the treated surface.
I agree with the answer from the other respondent, you'll have to try varoius methods for your particular cell type. If the usual flasks don't work, Sarstedt produces a line of tissue culture flasks specifically designed for suspension cells. They work fairly well and are economical.
Posted 30 July 2014 - 09:58 PM
Thanks very much, I'll try to place it vertical and see if it's alright.
Posted 17 August 2014 - 05:15 AM
We culture primordial germ cells in flasks and multi-well plates which are treated.
I'm not sure about orienting the flasks vertically - won't this reduce the rate of gas exchange and produce a much larger gradient of carbon dioxide/oxygen concentrations between the bottom of the medium and the surface? Maybe it's actually negligible; I don't know.