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Restriction and ligation time interval

ligation restriction

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#1 Raygoza

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Posted 11 July 2014 - 05:49 PM

Hi, is it better to do the ligation right after restriction? i wonder if there is the danger of ligation with other fragments in the case of pcr products because of sticky ends.

 

Thanks



#2 Trof

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Posted 12 July 2014 - 07:53 AM

As far as I know, sticky ends (as they are single stranded DNA) are more susceptible to degradation in time. It's better to use them fresh.


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