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Cell cycle determination

Staining Methods

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#1 moljul

moljul

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Posted 11 July 2014 - 04:05 AM

Hey folks,

 

I currently try to establish cell cycle measurements using FACS with cells stained with propidium iodide. To get a more accurate distribution of cells in a specific phase, I want to include BrdU staining or comparable method. Does any of you have experience with double-staining in cell cycle determination? Please let me know.

 

Thanks in advance,

moljul






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