Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

NSO cell culture

cholesterol dependence

  • Please log in to reply
1 reply to this topic

#1 sara.r

sara.r

    sara.r

  • Active Members
  • PipPipPipPipPip
  • 87 posts
1
Neutral

Posted 10 July 2014 - 09:22 AM

I have got some NSO  cells from a colleague and she gave me a medium which contained a specific cholesterol  supplement and told me that the NSO cells are dependent on the supplement for growth, however I  culture some cells in reqular medium with out the supplement and the cells look really happy after 3 passage. This is good news because the supplement is quite expensive but  we dont have much experience with NSO culture so I don't know if it is OK to culture cells without supplement.



#2 gfischer

gfischer

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 195 posts
9
Neutral

Posted 10 July 2014 - 01:07 PM

Probably not.  The cells may look superficially normal, but by culturing them under conditions other than standard, you're applying selection pressure.  Any results you get from experiments are not likely to be in line with properly cultured cells.


Above all things, if kindness is your king,
then heaven will be yours, before you meet your end




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.