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Aedes albopictus larvae cells (C6/36). Need Help, please.

c636 aedes albopictus

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#1 Thyago Leal

Thyago Leal

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Posted 09 July 2014 - 12:08 PM

Hi, everyone,

 

I have a problem with my C636 cells, today all of them seemed dead. 

Well, I'm using Leibovitz - 15 medium with no buffer system. Incubation at 28 °C with no CO2. 

And additional of conjugated Penicillin/Streptomycin and Anfotericin B.

 

I don't know what happened, the cells was just fine in one bottle, and then, after subculture to 2 new flasks: all dead. 

Besides, the medium was very pinky (indication of phenol red, meaning increase of pH).

 

 

I need some advice on the culture of C636 cells. A book, a reference, something. 

 

Thank you all for your support.

 

Best regards,

 

Thyago






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