I have a problem with my C636 cells, today all of them seemed dead.
Well, I'm using Leibovitz - 15 medium with no buffer system. Incubation at 28 °C with no CO2.
And additional of conjugated Penicillin/Streptomycin and Anfotericin B.
I don't know what happened, the cells was just fine in one bottle, and then, after subculture to 2 new flasks: all dead.
Besides, the medium was very pinky (indication of phenol red, meaning increase of pH).
I need some advice on the culture of C636 cells. A book, a reference, something.
Thank you all for your support.