Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

5' RLM RACE

TOTAL RNA PCR RNA PURIFICATION TSS Mapping

  • Please log in to reply
No replies to this topic

#1 Epigeneticist

Epigeneticist

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 97 posts
5
Neutral

Posted 09 July 2014 - 09:08 AM

1. Do you usually see an RNA pellet with the acid phenol-chloroform RNA purification step following CIP treatment? It is barely visible? I see a very small pellet after I spin down with ISOH and then it floats around when I wash with 70% EtOH. Not sure how big the pellet should be considering it is only 10ug of starting total RNA. 

 

2. Is it ok to use total RNA stored at -80C for 5' RLM RACE? Have anyone had success doing this? I know fresh would be the best but just wondering. 

 

Thanks!







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.