1. Do you usually see an RNA pellet with the acid phenol-chloroform RNA purification step following CIP treatment? It is barely visible? I see a very small pellet after I spin down with ISOH and then it floats around when I wash with 70% EtOH. Not sure how big the pellet should be considering it is only 10ug of starting total RNA.
2. Is it ok to use total RNA stored at -80C for 5' RLM RACE? Have anyone had success doing this? I know fresh would be the best but just wondering.