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Problem shrna cloning, bad sequencing results

shrna cloning sequencing

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#1 Finouu0106

Finouu0106

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Posted 09 July 2014 - 08:32 AM

Hello guys,

 

I'm trying to clone shRNA into pCL20c vector to transduce lentiviruses.

I get enough colonies to select several for miniprep and I check them by PCR before to send to sequence.

But everytime I've got a lot of mutation in my sequences ..

Anybody can help please ?

 

Thank you !



#2 bob1

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Excellent

Posted 09 July 2014 - 01:44 PM

Details needed...







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