We are interested in transfecting a CHO cell line to produce a recombinant protein.
We are planning to use the limiting dilution method in 96-well plates to select single-cell clones to be screened for expression and we are looking for a detailed protocol for this cell line. Specifically, we would also be interest to know the cloning efficiency with this cell line (i.e. the expected ratio between wells plated and clones obtained).
Edited by Debora, 09 July 2014 - 07:14 AM.