So today I was using the pipette gun, everything going normal, everything pipetted normally, until I got to the TPA. I used a 2ml pipette, but the TPA just didn't want to be sucked up! The level kept dropping until the liquid went back to the container it originally was in. I had warmed it up for 1 hr before I started pipetting, so I don't think this is an issue of it being too cold or something.
Any ideas on why this is happening?