Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

how to transport stem cells in suspension?


  • Please log in to reply
2 replies to this topic

#1 RLAD

RLAD

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 07 July 2014 - 04:14 PM

Hey, has anyone tried shipping overnight adherent mono-layered cultured cells in suspension. I used HIBERNATE®-E but seems like NSCs don't survive.

we can't ship cells frozen or in a flask because they have to be directly grafted in an animal because at the animal facility we can't culture cells.

best wishes



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,726 posts
399
Excellent

Posted 07 July 2014 - 06:14 PM

How long can you keep them in suspension before they die?  It might well be that the cells require attachment for survival. 

 

You could try using a low binding surface (just google it for your favourite brands), they should be better for keeping the cells in suspension than regular cell culture treated flasks.



#3 RLAD

RLAD

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 July 2014 - 09:53 AM

Thats a good idea, I will try it out






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.