2 replies to this topic

[RLAD]

Hey, has anyone tried shipping overnight adherent mono-layered cultured cells in suspension. I used HIBERNATE®-E but seems like NSCs don't survive.

we can't ship cells frozen or in a flask because they have to be directly grafted in an animal because at the animal facility we can't culture cells.

best wishes

[bob1]

How long can you keep them in suspension before they die?  It might well be that the cells require attachment for survival. 

 

You could try using a low binding surface (just google it for your favourite brands), they should be better for keeping the cells in suspension than regular cell culture treated flasks.

[RLAD]

Thats a good idea, I will try it out