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TET off plasmid recommendations

TET off doxycyline

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#1 ramblingrat



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Posted 07 July 2014 - 01:28 PM

Hi everyone,


I am trying to establish a tet off system for my gene of interest, on a lentiviral backbone suitable for 3rd generation packaging. 


Can anyone recommend:

1.) Doxy-system newbie information literature :o)

2.) A good backbone plasmid for that purpose?


Thank you very much!!!



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