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TET off plasmid recommendations

TET off doxycyline

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#1 ramblingrat

ramblingrat

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Posted 07 July 2014 - 01:28 PM

Hi everyone,

 

I am trying to establish a tet off system for my gene of interest, on a lentiviral backbone suitable for 3rd generation packaging. 

 

Can anyone recommend:

1.) Doxy-system newbie information literature :o)

2.) A good backbone plasmid for that purpose?

 

Thank you very much!!!

 

R.






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