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Extra- and intra-cellular ATP levels in HepG2

ATP cell culture intracellular extracellular

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#1 Giusta

Giusta

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Posted 07 July 2014 - 04:58 AM

Hi to everybody, 

I would like to measure intra- and extra-cellular levels of ATP in HepG2 cell culture.

 

Is it possible to evaluate the amount of Intracellular ATP through Cell-titerGLo?

 

How can I evaluate only the extra-cellular amount? Can i use apyrase and observe the difference with or without  extra-cellular ATP depletion? Do I have to use apyrase together with ATP channels inhibitors?

 

Thanks in advance







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