Hi to everybody,
I would like to measure intra- and extra-cellular levels of ATP in HepG2 cell culture.
Is it possible to evaluate the amount of Intracellular ATP through Cell-titerGLo?
How can I evaluate only the extra-cellular amount? Can i use apyrase and observe the difference with or without extra-cellular ATP depletion? Do I have to use apyrase together with ATP channels inhibitors?
Thanks in advance