Hi all, I have a question regarding what type of statistical test to use, I haven't done stats for many many years and need some help!
I'm currently working on a project which is assessing the best culture conditions to grow mesenchymal stem cells in. The experiment runs as follows:
We have 4 media conditions:
1) DMEM with 5% FBS
2) DMEM with 5% FBS + FGF
3) DMEM with 10% FBS
4) DMEM with 10% FBS + FGF.
Each of these conditions are being tested for growth in:
1) 21% oxygen
2) 2% oxygen
3) 2% oxygen enclosed environment
We have 6 flasks in each set (except for the 10% FBS + FGF group being grown in the 2% oxygen group - we lost 2 of those to infection, and we lost all of the 10% flasks in the 2% oxygen enclosed environment) so in total we have 60 flasks.
The only data I have to work with is % estimated confluency for some of these flasks, % viability and colony counts. My question was, what sort of test do I need to do on these? I'm assuming it would be chi-squared as I'm testing for a difference between counts, but because I have so many groups would I go for an ANOVA test?? I haven't done this in so long and any help would be much appreciated!
We're seeing what effect different concentrations of FBS have, what effect FGF (or absence thereof) has and finally which oxygen conditions are best for growth. Also is anyone using the program Prism 6? I'm just trying to get to grips with it and can't decide which table to use, currently it's between using 'Grouped Tables' and 'Contingency Tables'
Many thanks in advance!