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Tissue Preservation

tissue preservation dna preservation dna extraction methanol methanol anhydrous

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#1 dolph22



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Posted 02 July 2014 - 11:24 AM

I generally preserve specimens (whole fish) in ethanol to preserve tissue for DNA extraction to use in PCR reactions. I am out of ethanol at the moment but can borrow some Methanol Anhydrous (ultra pure grade). Will this preserve the tissue identically to ethanol, for purposes of DNA extraction?

#2 hobglobin


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Posted 02 July 2014 - 11:51 AM

As far as I know methanol works as good as ethanol, and even penetrates tissues better and faster (lower molecular weight). The only problem will be the toxicity of methanol, so you have to work carefully.

No idea if pure or diluted methanol is better.

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that did belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

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