I am posting here for the first time. I am trying to determine the mechanism of an enzyme catalyzed reaction. It has two substrates. I found that the enzyme is inhibited by one of its substrate and follows a compulsory order ternary complex mechanism.
I am quite confident about my data but my supervisor thinks I should do product inhibition studies in order to confirm this mechanism. I have no experience with this and I am not sure if this will be a correct way to do it because substrate inhibition will make it more complicated.
Any suggestions will be very helpful and I thank you in advance.