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3T3 fibroblast

feeder cells irradiated mitomycin C oral keratinocytes

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#1 roxx_gurl

roxx_gurl

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Posted 01 July 2014 - 10:50 PM

Hi

 

Is there anyone here has experience on using 3T3 feeder cells (fibroblast) to grow keratinocytes? and has experience on using either mitomycin  C treated or irradiated? Which one is the best in term of time consuming and re-use the feeder cell?

 

If I am thinking of purchasing, which cell lines of feeder layer is recommended?

 

Thanks






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