96 Well PCR clean up
Posted 29 June 2004 - 09:55 PM
I'm pretty new here but I like the look of this place, so sorry if I've broken protocol.
What I'm looking for is a good product that enables 96 well cleanup of PCR products for the purposes of sequencing.
I would also rather use a centrifugation step in this process rather than using a vacuum pump, any suggestions.
Posted 29 June 2004 - 10:14 PM
Posted 11 March 2012 - 04:09 PM
-High-Throughput PCR Clean-Up / Gel Extraction-The 96-Well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unicorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, litigation, PCR and sequencing reactions.
- Sample Size: Up to 50ul of PCR product
- Format: 96-Well Plates
- Operations: Centrifuge / Vacuum Manifold
- Binding Capacity: 10mg per well
- DNA Size: 50bp - 10kb
- Recovery: 80-90% for Gel Extraction / 90-95% for PCR Clean-Up
- Operation Time: 30 minutes for PCR Clean-Up / 40 minutes for Gel Extraction