2 replies to this topic

[Schrodinger's Cat]

Hi guys,
I'm pretty new here but I like the look of this place, so sorry if I've broken protocol.

What I'm looking for is a good product that enables 96 well cleanup of PCR products for the purposes of sequencing.
I would also rather use a centrifugation step in this process rather than using a vacuum pump, any suggestions.
Cheers guys.

[Schrodinger's Cat]

Oh yeah my pcr products are from about 500bp to 900bp, if that information helps anyone to help me out.
Cheers.

[parasuis]

I use the NucleoFast 96 PCR by Macherey-Nagel (http://www.macherey-nagel.com) and it works like a charm. I use their vacuum manifold, but you can also stick it in a centrifuge.