Hi, I have a query for anyone who does their own sequencing gels (in the old-school way!) or uses large-format hot denaturing PAGE gels for single-nucleotide resolution of primer extension products. My query is what company made your sequencing/electrophoresis rig? And what catalogue number, if you know?
The lab next door to us has a BioRad rig, and I have been using it to separate radiolabelled primer extension reaction products. We want to buy our own but BioRad doesn't make it anymore and I am having trouble sourcing a similar set up. Our current gels are 20x50cm and we want something of a similar size, that can go up to 55 or 60watts and reach 45 or 50C temperatures to get good resolution.
Is there anyone out there who has a similar rig that they can tell me about?!