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Is it possible to perform an MS analysis on an EMSA?

MALDI MALDI-TOF EMSA

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#1 Antonio Sillo Rosato

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Posted 30 June 2014 - 09:16 PM

I performed some EMSA assays with very puzzling results. Although I'm sure that in the shifts there is my protein, I'd like to better characterize them.
Is it possible to excise the band of interest, perform on membrane tryptic digestion and then spot the matrix for the MALDI? 
I've used 5 µg of protein extract and 30 or 100 fmol of biotinylated DNA and a positively charged nylon membrane for the blotting.



#2 neuron

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Posted 30 June 2014 - 10:39 PM

I found the similar question in achieves-

 

http://www.protocol-online.org/biology-forums/posts/23374.html 

 

It should be possible, I don't know much details of EMSA, but here is one paper, it may be related to your work-

 

http://utsa.edu/chem...tt/EMSA-3DE.pdf


Edited by neuron, 30 June 2014 - 10:42 PM.


#3 Antonio Sillo Rosato

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Posted 01 July 2014 - 09:34 PM

thanks






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