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How to run genscan on a large file

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#1 Garima



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Posted 30 June 2014 - 08:45 PM

Hi all,

I am currently working on an assembled eukaryotic genome. The file size is 2.5 GB. I have read that people use genscan for de-novo gene prediction. Currently i am unable to figure out how as genscan does not work well with multifasta and large files. I need to produce a .gff file. I used a perl script that was available i.e run_genscan_gff.pl. But that script also encounters the same problem of large file. How does everyone else do this prediction using Genscan?? Any help would be useful right now.


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