I am currently performing a qPCR on a LightCycler 480 in the way of melt curve analysis. I get a peak at the appropriate temperature but no there is no amplification on the Ct aspect. Can this be regarded as a sample that has the virus present? Why does it do this? Does it need to be re-ran/re-extracted?
Thank you in advance for any assistance.
Edited by BeepJeep, 30 June 2014 - 07:30 PM.