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contamination


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#1 solcole

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Posted 28 June 2014 - 09:08 AM

Hi,

 

I had two dishes of iPS cells. In one of the dishes, I observed a mold-like colony which looks like a fuzzy ball with small protrusions all around and the iPS cells were dying. I discarded it and now the other dish had these vibrating small black dots (see attached image), many of them. The cells in this dish is also dying. I use pen-strep in the medium and so I don't think it is a cell-wall bacteria. Any idea what kind of contamination this is? Mycoplasma or mold or something else?

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  • what is this.jpg


#2 Tabaluga

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Posted 28 June 2014 - 10:03 AM

Are they really moving or just vibrating with the fluid (then it could be debris) ?

Anyway, have a look at these threads too:

 

http://www.protocol-...ots#entry138932

http://www.protocol-...-helpful-paper/


Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#3 solcole

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Posted 28 June 2014 - 10:15 AM

They don't seem to be moving with the media...they are vibrating on their own. And there are too many black dots; usually the cells do not have this much of debris. Coupled with the fact that the cells are dying, this is highly likely a contaminant but im not sure what type?



#4 Tabaluga

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Posted 28 June 2014 - 10:44 AM

Did you take a look at the threads ? It could be the discussed Achromobacter.

BTW, mycoplasma are not normally visible.


Edited by Tabaluga, 28 June 2014 - 10:48 AM.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#5 Phil Geis

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Posted 29 June 2014 - 08:51 AM

"Vibration" per se could be Brownian and probably isn't that relevant.  If it;s bacterial, there's nothing to say it's Achormobacter over any other.  Try to culture the stuff.






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