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Beta Agarase 1 Gel Extraction

Gel extraction

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#1 hogthehedge



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Posted 25 June 2014 - 05:39 PM

Has anyone ever tried beta-agarase 1 by NEB for gel extraction of couple hundred bp DNA? How do you know if it has worked? When I complete the digestion and put the tubes on ice to try and extract the DNA, the agarose in it is polymerizing into a gel again. Isn't this supposed to not happen?

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