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How to preserve bacteria at 4 degree

microbiology

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#1 Celz

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Posted 24 June 2014 - 12:47 AM

Dear all, 

 

Does anyone know is there any method can use to preserve bacteria at 4 deg. cel? tq. 



#2 bob1

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Posted 24 June 2014 - 02:01 AM

Nothing lasts forever, but a stab culture will be about as good as you will get.



#3 Adriana Reis

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Posted 01 August 2014 - 03:25 AM

Since I have no 80C I keep making stock cultures of my bugs every now and then and thats how I preserve them in the fridge.



#4 Trof

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Posted 02 August 2014 - 02:48 AM

I would like to make my cells survive for a relatively short period (weeks) between making more preps, but without loosing the plasmid (~11 kb) and without recombination of it (retroviral vector). I was storing the remains of liquid cultures in a fridge for a day or over a weekend, but this is a bit longer time. I will do a -70 stock too, for a long-term storage, but I don't want to use it after few weeks again.

 

I never done a stab culture before, can I just stab a normal LB agar I use (with ampicilin) or do I need a special medium for that? I suppose for a few weeks it would probably survive on the plates where they were cultivated, but at the time I picked them they were already making satellite colonies, so that woudn't be a good idea.


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#5 bob1

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Posted 02 August 2014 - 03:30 AM

A stab should last at least a couple of weeks, but like any culture kept at 4, it will degrade over time, so there is no guarantee that it will work well. I would use the normal growth medium + agar for the stab.







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