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Methanol concentration for cells?

methanol cell culture

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4 replies to this topic

#1 medibio

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Posted 23 June 2014 - 02:32 AM

Hello,

 

I will solve a drug to use methanol for HT-29 cell line. Final concentration for methanol is 0,3 %. Is it toxic for the cell line? Can I use the drug at 0,3 % final concentration?



#2 bob1

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Posted 23 June 2014 - 02:22 PM

The answer is "Maybe" 0.3% will probably be fine, but it depends on the cell line a lot.  Note that if the drug is soluble in methanol, it will also be soluble in ethanol, which might be less toxic.



#3 medibio

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Posted 25 June 2014 - 07:00 AM

 

 

if the drug is soluble in methanol, it will also be soluble in ethanol

Is it accurate? If Ok, I can use EtOH.



#4 bob1

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Posted 26 June 2014 - 12:34 AM

Methanol and Ethanol are very similar chemicals - the only difference is a CH3 group, so they behave in the same fashion as other members of the alcohol group.



#5 mlomonaco

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Posted 09 July 2014 - 04:18 PM

I recommend a titration of your selected solvent and assess toxicity by yor
favorite assay. Alternatively, many drugs are available as water soluble salts or
in pharmaceutical encapsulations (eg., PEG or





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