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Plasmid storage conditions


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3 replies to this topic

#1 science noob

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Posted 22 June 2014 - 11:56 PM

Is it still safe to use a plasmid kept in water in the 4C fridge for 1 year?



#2 bob1

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Posted 23 June 2014 - 12:02 AM

Safe for what? - you shouldn't catch any illnesses from it so long as it isn't visibly growing something,

 

The only way to tell if it is useable is to run some on a gel and look at its integrity.

 

Depending on application, you might be better off transforming and doing a fresh prep.



#3 mdfenko

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Posted 23 June 2014 - 05:02 AM

yes, check it's integrity on a gel. it may have been acid hydrolyzed by the water (after absorbing co2 from the air).


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#4 phage434

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Posted 23 June 2014 - 05:05 AM

If it was stored in TE rather than water, I'd say it is likely fine. If in water, then I would have doubts. Almost certainly you could transform it, which is what I would do immediately, then do a new prep.






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