Is it still safe to use a plasmid kept in water in the 4C fridge for 1 year?
Plasmid storage conditions
Posted 23 June 2014 - 12:02 AM
Safe for what? - you shouldn't catch any illnesses from it so long as it isn't visibly growing something,
The only way to tell if it is useable is to run some on a gel and look at its integrity.
Depending on application, you might be better off transforming and doing a fresh prep.
Posted 23 June 2014 - 05:02 AM
yes, check it's integrity on a gel. it may have been acid hydrolyzed by the water (after absorbing co2 from the air).
genius does what it must
i do what i get paid to do
Posted 23 June 2014 - 05:05 AM
If it was stored in TE rather than water, I'd say it is likely fine. If in water, then I would have doubts. Almost certainly you could transform it, which is what I would do immediately, then do a new prep.