Can anyone offer any advice or suggestions for isolating and identifying microorganisms from soil?
So far I was thinking of stirring the soil in a solution like 1X PBS and then streaking onto a variety of plates such as PEA, DES ,McConkey and TSA.
Then isolating and sub culturing and eventually identifying.
The identification part, I am not sure if I should try biochemical tests or 16S sequencing??
Our lab was traditionally chemistry synthesis but now we are becoming interdisciplinary with microbiology so we don't have PCR/sequencing capabilities as yet or biochemical identification assays, so cost would be a factor as these things need to be purchased or outsourced.
Any suggestions of advice would be great.