I would like to make a digest with BspEI and XmaI , their buffers are not compatible, so I need to do a sequential digest.
Now can I just use XmaI in the specific buffer (50mM NaCl) and after this reaction is done just add some of the BspeI buffer (100mM potassium acetate)?
Or the other way around.
I read on the NEB website somewhere you can do a sequential restriction reaction using the lowest salt concentration first and than the highest one.
Or are they not compatible at all?