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why eea(early endosome) is stained to nuclei?


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#1 flightyoon



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Posted 18 June 2014 - 01:19 AM

Hello guys~


i attached the picture and have a some problem.


i stained EEA1(early endosome marker). however there are many spots in the nuclei. it shouldn't be observed i think.


is it normal? or should i change some protocol?


this is my short protocol


used -20 degree methanol for fixation and permeablization 10minute.


blocked with 3%BSA/PBS 30minute RT


stained EEA1 diluted in 3%BSA/PBS 1hour RT with low speed shaking.


wash 3times PBS and confocal microscopy


please reply for my topic for my work. HHHHELP Me~~~

Attached Files

  • Attached File  wqw.bmp   94.97KB   237 downloads

Edited by flightyoon, 18 June 2014 - 01:22 AM.

Also tagged with one or more of these keywords: eea

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