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why eea(early endosome) is stained to nuclei?

eea

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#1 flightyoon

flightyoon

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Posted 18 June 2014 - 01:19 AM

Hello guys~

 

i attached the picture and have a some problem.

 

i stained EEA1(early endosome marker). however there are many spots in the nuclei. it shouldn't be observed i think.

 

is it normal? or should i change some protocol?

 

this is my short protocol

 

used -20 degree methanol for fixation and permeablization 10minute.

 

blocked with 3%BSA/PBS 30minute RT

 

stained EEA1 diluted in 3%BSA/PBS 1hour RT with low speed shaking.

 

wash 3times PBS and confocal microscopy

 

please reply for my topic for my work. HHHHELP Me~~~

Attached Files

  • Attached File  wqw.bmp   94.97KB   25 downloads

Edited by flightyoon, 18 June 2014 - 01:22 AM.






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