hello
i've been making SDs-page gels both 12% and 16% for a while now but i had a problem after staining the gel with either silver of coomassie stain which is that a line appears at the lower end of the gel and the problem is that some important bands appear around that mwt and can't be seen because of this random line i use a similar recipe to this one http://mcardle.oncol...preparation.pdf for the gel so can anyone help me in solving this problem
thank you