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Need a clarification


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#1 Gnanasagar

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Posted 17 June 2014 - 05:17 PM

Why do you have to passage a cell line? Why not use it as it is?



#2 bob1

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Posted 18 June 2014 - 12:06 AM

I'm not entirely sure of the question - use it as is?  How do you think it got to where it is?



#3 Tabaluga

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Posted 18 June 2014 - 01:05 AM

In short: because if you didn't passage cells, they would grow more and more confluent, and over time nutrients from the medium are consumed, until there is not enough nutrients left, and the cells would die.


Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 





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