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autoclaving guide


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#1 bioke

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Posted 17 June 2014 - 08:12 AM

Hallo,

 

Has anyone a good guide on autoclaving?

 

Some people state its 15 minutes at 120°C , some state its 20 minutes at 120°C, other state its 15 minutes at 121°C....

 

I can google it myself and I am able to find those date, but I cant seem to find any good "evidence" or a nice guide/book on this subject.

 

Anyone a good link?



#2 lsek

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Posted 18 June 2014 - 12:49 AM

Hi,

 

I followed Hirayama autoclave manual. Online manual can be downloaded from this link (http://sydney.edu.au...ries_manual.pdf).

 

For a more detailed explanation you can start with (https://medschool.va...toclave-service) that showed liquid volume versus autoclaving time needed to achieve 1 to 10^6 Serility Assurance Level (SAL; see wikipedia http://en.wikipedia....assurance_level).

 

I've also found a paper regarding "top 10 considerations when validating autoclave" (http://www.pharmout...._validation.pdf)

 

I would just stick to the Hirayama or whichever brand of autoclave you are using because most of the time, we prepare our solutions in "sterile" distilled/deionized water. No need to really go for high SAL.

 

P.S. 121°C at 15 min is the minimum (and also suitable for media that can't withstand longer sterilization).

 

Cheers


Edited by lsek, 18 June 2014 - 12:52 AM.

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