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autoclaving guide

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#1 bioke



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Posted 17 June 2014 - 08:12 AM



Has anyone a good guide on autoclaving?


Some people state its 15 minutes at 120°C , some state its 20 minutes at 120°C, other state its 15 minutes at 121°C....


I can google it myself and I am able to find those date, but I cant seem to find any good "evidence" or a nice guide/book on this subject.


Anyone a good link?

#2 lsek



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Posted 18 June 2014 - 12:49 AM



I followed Hirayama autoclave manual. Online manual can be downloaded from this link (http://sydney.edu.au...ries_manual.pdf).


For a more detailed explanation you can start with (https://medschool.va...toclave-service) that showed liquid volume versus autoclaving time needed to achieve 1 to 10^6 Serility Assurance Level (SAL; see wikipedia http://en.wikipedia....assurance_level).


I've also found a paper regarding "top 10 considerations when validating autoclave" (http://www.pharmout...._validation.pdf)


I would just stick to the Hirayama or whichever brand of autoclave you are using because most of the time, we prepare our solutions in "sterile" distilled/deionized water. No need to really go for high SAL.


P.S. 121°C at 15 min is the minimum (and also suitable for media that can't withstand longer sterilization).



Edited by lsek, 18 June 2014 - 12:52 AM.


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