footprinting & gelshift
Posted 28 June 2004 - 01:51 AM
I have checked the Amersham website, and it says that
"The synthetic polymer poly(dI-dC)¡Ppoly(dI-dC) is useful in DNA-binding protein studies (1-4) because it is sufficiently like cellular DNA to soak up non-specific binding proteins, yet sufficiently different to avoid inadvertent binding of the specific proteins of interest."
But why Poly(dI-dC)¡Ppoly(dI-dC) can bind with other non-specific protein?
Posted 28 June 2004 - 08:52 AM
To maximize its effectiveness, the competitor DNA must be added to the reaction along with the protein prior to the labeled DNA target.
Posted 21 May 2009 - 11:05 AM