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Temperature sensitivity of Plasmids


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#1 Raygoza

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Posted 15 June 2014 - 07:28 PM

Hi, i'm working concurrently in  two projects involving plasmids that are temperature sensitive, one of them is said to replicate at 28ºC and the other at 30º C , However, we have only two incubators at the lab and i couldn't take both of them and is making things difficult for me. Could I grow the bacteria of one plasmid in the other's temperature? is 2º a catastrophic difference for the plasmids? thanks a lot it will save me a lot of time or at least will help me explain other people why i would be using the two incubators.

 

 

 

 



#2 pito

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Posted 16 June 2014 - 01:38 AM

Hi, i'm working concurrently in  two projects involving plasmids that are temperature sensitive, one of them is said to replicate at 28ºC and the other at 30º C , However, we have only two incubators at the lab and i couldn't take both of them and is making things difficult for me. Could I grow the bacteria of one plasmid in the other's temperature? is 2º a catastrophic difference for the plasmids? thanks a lot it will save me a lot of time or at least will help me explain other people why i would be using the two incubators.

 

 

 

 

To be honest: I wonder how you are going to checkt that 2°C difference!

Many incubators shift between (lets say) 35 and 38°C when they are set at 37°C...

And thats not even talking about people opening them to take stuff out and so on...

 

28 or 30°C will be fine for both plasmids...

 

(I am guessing the plasmid has a heat sensitive origin of replication? In most cases: this is more something that has a certain boundary, like for example: "will not replicate anymore abote 40°C" or something like that, but being sensitive to a specific certain temperature for replication itself is a bit weird.. it seems to be more an ideal temperature that you mean) 

You are sure its the plasmid and not the bacterium that is heat sensitive?


If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.




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