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DMSO in restriction digest

restrict dmso

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#1 Raygoza

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Posted 14 June 2014 - 10:04 PM

Hi, i was wondering if someone has tried adding dmso to a restriction digest to reduce the number of supercoiled plasmids and therefore help with the restriction efficiency? does this sound reasonable? would dmso rather interfere than help?

 

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#2 bob1

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Posted 14 June 2014 - 11:47 PM

It isn't necessary - you can take a prep that is 100% supercoiled and digest it effectively with all the common restriction enzymes that I have worked with.







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